Gruppe Posthaus

Horst Posthaus
Prof. Horst Posthaus

The focus of our research is the interaction of bacterial pathogens with their animal hosts. We are particularly interested in the mechanism of action of bacterial exotoxins on target cells. In light of the increasing problem of antibiotic resistance, there is a need for novel approaches to treat bacterial infections in humans and animals, e.g. vaccinations or therapeutics targeting bacterial effector proteins.

Many proteins secreted by pathogenic bacteria are toxins that act on specific target cells in the body of the host.  To develop novel therapies, it is crucial to understand the mechanisms underlying the targeted attack exerted by these powerful bacterial weapons.

Pathogenic clostridia, such as C. perfringens, C. botulinum, C. chauvoei, C. septicum, etc., cause severe diseases in animals and humans. The pathogenesis of many of these diseases is poorly understood. Clostridia produce a wide range of exotoxins to manipulate their hosts. Amongst these toxins are some of the most potent bacterial exotoxins known, such as botulinum or tetanus toxin.

We currently focus on the cellular and molecular effects of Clostridium perfringens beta-toxin on target cells. The toxin is known as the essential virulence factor of C. perfringens type C strains, which cause a fatal acute necrotizing enteritis in pigs and other hosts, including humans. The toxin belongs to the family of hemolysin-beta-pore-forming toxins and forms small pores in the membrane of target cells. This leads to cell death and the associated lesions in affected organs.

We previously identified endothelial cells as primary targets for this toxin and established cell culture based in vitro models to investigate the molecular mechanisms of beta-toxin induced endothelial damage. Besides this, we perform studies on the control of C. perfringens type C in Swiss pig herds. Our research has been funded by the Swiss National Science Foundation (SNF) and the Federal Food Safety and Veterinary Office (FSVO).

Besides our primary research topic we offer support for research groups at our faculty and outside as part of our COMPATH platform.

Gruppenleiter

Mitarbeitende

ausgewählte Manuskripte aus unserer Forschung

  • Bruggisser, J., Tarek, B., Wyder, M., Witz, G., Enzmann, G., Deutsch, U., Engelhardt, B., Posthaus, H. Cell-specific targeting by Clostridium perfringens β-toxin unraveled: the role of CD31 as the toxin receptor. BioRxvi, 2019, https://doi.org/10.1101/787242
  • Richard, O. K., Grahofer, A., Nathues, H., and Posthaus, H. (2019) Vaccination against Clostridium perfringens type C enteritis in pigs: a field study using an adapted vaccination scheme. Porcine Health Manag 5, 20 https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-019-0127-8
  • Richard, O. K., Springer, S., Finzel, J., Theuss, T., Wyder, M., Vidondo, B., and Posthaus, H. (2019) Application of an Endothelial Cell Culture Assay for the Detection of Neutralizing Anti-Clostridium Perfringens Beta-Toxin Antibodies in a Porcine Vaccination Trial. Toxins 11 https://www.mdpi.com/2072-6651/11/4/225
  • Thiel, A., Mogel, H., Bruggisser, J., Baumann, A., Wyder, M., Stoffel, M. H., Summerfield, A., and Posthaus, H. (2017) Effect of Clostridium perfringens beta-Toxin on Platelets. Toxins 9, https://www.mdpi.com/2072-6651/9/10/336
  • Roos, S., Wyder, M., Candi, A., Regenscheit, N., Nathues, C., van Immerseel, F., and Posthaus, H. (2015) Binding studies on isolated porcine small intestinal mucosa and in vitro toxicity studies reveal lack of effect of C. perfringens beta-toxin on the porcine intestinal epithelium. Toxins 7, 1235-1252 https://www.mdpi.com/2072-6651/7/4/1235
  • Schumacher, V. L., Martel, A., Pasmans, F., Van Immerseel, F., and Posthaus, H. (2013) Endothelial binding of beta toxin to small intestinal mucosal endothelial cells in early stages of experimentally induced Clostridium perfringens type C enteritis in pigs. Veterinary pathology 50, 626-629 https://doi.org/10.1177/0300985812461362

Eine vollständige Publikationenliste finden Sie unter NCBI.